LC-MS/MS-Based Assay for Free and Deconjugated Testosterone and Epitestosterone in Rat Urine and Serum
نویسندگان
چکیده
The 2014 World Anti-Doping Agency (WADA) Prohibited List shows testosterone and epitestosterone listed as class S1.1a and S1.1b, with its use banned in and out of competition [1]. Doping with testosterone is suspected if the urinary ratio of testosterone over epitestosterone (T/E) exceeds 4 [2]. According to the 2012 (WADA) laboratory statistics, elevated testosterone based on the testosterone over epitestosterone (T/E) ratio accounts for 55.5% of atypical findings within the anabolic agents’ drug class from a total of 2,279 for all anabolic agents, which constitute half of all adverse and atypical findings [3]. This T/E ratio approach to testing for testosterone abuse is only accurate if testosterone and epitestosterone are metabolised at the same rate and no other external factors or inter individual factors would alter the metabolism of these steroids [4].
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